Institute of Molecular and Cell Biology, National University of Singapore, 10 Kent Ridge Crescent, 0511, Singapore, Republic of Singapore.
Plant Cell Rep. 1990 Aug;9(4):195-8. doi: 10.1007/BF00232178.
The presence of 1-10 μM aminoethoxyvinylglycine (AVG) or 5-30 μM AgNO3 markedly enhanced shoot regeneration from cotyledon and hypocotyl cultures of eight recalcitrant Brassica campestris and B. juncea genotypes tested. Expiants of B. campestris ssp. chinensis and ssp. parachinensis grown with a high AVG concentration (20 μM), regenerated poorly. All cytokinins tested were equally effective in promoting shoot formation, except that kinetin was inhibitory to shoot regeneration from hypocotyls of B. campestris ssp. pekinensis (cv. Wong Bok). Both AgNO3 and AVG had no effect on percent rooting and number of roots per rooted cutting of Wong Bok, White Sun and Leaf Heading, but AgNO3 was inhibitory to rooting of India Mustard. However, root elongation of all cuttings was markedly inhibited by AVG at concentrations of 5 and 10 μM.
1-10 μM 氨基乙氧基乙烯基甘氨酸（AVG）或 5-30 μM 硝酸银的存在显著增强了从 8 种抗性 Brassica campestris 和 B. juncea 基因型的子叶和下胚轴培养物中再生芽。用高浓度 AVG（20 μM）生长的 Brassica campestris ssp. chinensis 和 ssp. parachinensis 的外植体再生能力较差。所有测试的细胞分裂素在促进芽形成方面同样有效，除了激动素对 Brassica campestris ssp. pekinensis（cv. Wong Bok）下胚轴的芽再生具有抑制作用。AgNO3 和 AVG 对 Wong Bok、White Sun 和 Leaf Heading 的生根百分率和每根生根插条的根数量均无影响，但 AgNO3 对印度芥菜的生根有抑制作用。然而，AVG 在 5 和 10 μM 浓度下显著抑制所有插条的根伸长。
