Department of Plant Genetics, The Weizmann Institute of Science, 76100, Rehovot, Israel.
Plant Cell Rep. 1988 Oct;7(6):403-6. doi: 10.1007/BF00269523.
Ethylene release by potato shoots cultured in closed boxes was suppressed by the addition of silver thiosulfate to the culture medium. Shoots cultured in the presence of silver thiosulfate produced appreciably more tissue and the yield of protoplasts per unit tissue mass was vastly increased, resulting in an 8 fold increase of protoplast yield per shoot. Exposure of pricked leaves to macerating enzymes facilitated ethylene generation. Leaves of shoots which were previously cultured in silver thiosulfate containing medium generated much less ethylene than leaves from control shoots and this generation could be further reduced by the addition of acetylsalicylic acid during maceration. The capability of polyethylene glycol treated potato protoplasts to produce microcalli was vastly increased by the addition of silver thiosulfate during exposure of protoplasts to Ca(NO3)2 following the polyethylene glycol treatment. Similarly, when a plasmid (pCAP212) containing an expressible gene for chloramphenicol acetyltransferase was introduced into potato protoplasts through a polyethylene glycol treatment, the transient expression of acetyltransferase was very much increased by the addition of a short incubation of the protoplasts with silver thiosulfate.
在密闭箱中培养的土豆苗所释放的乙烯，可通过在培养基中添加硫代硫酸银来抑制。在硫代硫酸银存在的情况下培养的土豆苗会产生大量的组织，单位组织质量的原生质体产量也大大增加，导致每个土豆苗的原生质体产量增加了 8 倍。刺伤的叶片暴露于捣碎酶中会促进乙烯的产生。与对照植株的叶片相比，先前在含有硫代硫酸银的培养基中培养的植株叶片产生的乙烯要少得多，并且在捣碎过程中添加乙酰水杨酸可以进一步减少乙烯的产生。在聚乙二醇处理后，将原生质体暴露于 Ca（NO3）2 时，添加硫代硫酸银可大大增加经聚乙二醇处理的土豆原生质体产生微愈伤组织的能力。同样，当通过聚乙二醇处理将含有可表达氯霉素乙酰转移酶基因的质粒（pCAP212）导入土豆原生质体时，通过短时间与硫代硫酸银孵育原生质体，可大大增加乙酰转移酶的瞬时表达。
