Cambridge Laboratory, JI Centre for Plant Science Research, Colney Lane, NR4 7UJ, Norwich, UK.
Theor Appl Genet. 1990 Jun;79(6):813-6. doi: 10.1007/BF00224250.
The agronomic performance of 9 doubled haploid (DH) lines of Chinese Spring, 6 DH lines of Hope, 14 DH lines of the single chromosome substitution line Chinese Spring (Hope 5 A) and their respective parents was analyzed under field conditions. Seventeen Chinese Spring DH lines derived from wheat x Hordeum bulbosum crosses were also included for comparison. No significant variation was detected in either population of Chinese Spring DH lines and neither DH population differed from its parent. The Hope DH lines differed significantly for tiller biomass, spikelet number per ear, ear grain weight and 50-grain weight. However, all the variation could be attributed to the poor performance of only one line. Chinese Spring (Hope 5 A) DH lines showed significant variation for ear emergence time, but this was probably due to genetic heterogeneity in the parental stock. Overall, the results suggest that most DH lines produced by the wheat x maize method resemble their wheat parent, and that the variation induced in DH production is likely to be similar to that found in DHs from wheat x Hordeum bulbosum crosses.
在田间条件下,分析了 9 个中国春双倍单倍体 (DH) 系、6 个 Hope DH 系、14 个中国春单染色体代换系 (Hope 5A) 及其各自亲本的农艺性能。还包括了来自小麦 x 大麦杂交的 17 个中国春 DH 系进行比较。在两个中国春 DH 系群体中均未检测到显著变异,且两个 DH 系群体与其亲本均无差异。Hope DH 系在分蘖生物量、小穗数/穗、穗粒重和 50 粒重方面存在显著差异。然而,所有的变异都可以归因于仅一条线的不良表现。中国春 (Hope 5A) DH 系的穗出现时间存在显著差异,但这可能是由于亲本材料的遗传异质性所致。总体而言,结果表明,通过小麦 x 玉米方法产生的大多数 DH 系与它们的小麦亲本相似,并且在 DH 生产中诱导的变异可能与小麦 x 大麦杂交产生的 DH 中的变异相似。