Heterosis Laboratory, National Institute of Agrobiological Resources, Kan-non-dai 2-1-2, Tsukuba, 305, Ibaraki, Japan.
Plant Cell Rep. 1989 May;8(5):263-6. doi: 10.1007/BF00274125.
Four Japanese wheat varieties, three crossable and one non-crossable with Hordeum bulbosum, were pollinated with maize pollen of 5 genotypes. By the application of 2,4-dichlorophenoxyacetic acid after pollination, embryos kept developing on wheat plants until 14 days after pollination. The frequency of embryo formation was significantly different among the maize genotypes, varying from 18.0% to 31.9%, but not among the wheat varieties. By bagging spikes with flag leaves the frequency of embryo formation was increased by about 7%. Ten- to twelve-day-old embryos gave higher frequencies of plant formation (83.6%) than 14-day-old embryos(50.0%). All 6 regenerated plants investigated cytologically were found to be haploid. Twelve of the 14 colchicine-treated plants produced florets setting seeds. The overall efficiency of our procedure is considered to be higher than that reported by Laurie and Bennett (1988).
四个日本小麦品种，三个可与 Hordeum bulbosum 杂交，一个不可与 Hordeum bulbosum 杂交，用 5 种基因型的玉米花粉授粉。授粉后应用 2,4-二氯苯氧乙酸，使胚胎在小麦植株上继续发育，直到授粉后 14 天。玉米基因型之间胚胎形成的频率有显著差异，从 18.0%到 31.9%不等，但小麦品种之间没有差异。用旗叶套袋可以将胚胎形成的频率提高约 7%。10 到 12 天龄的胚胎比 14 天龄的胚胎（50.0%）形成植株的频率更高（83.6%）。所有 6 个经过细胞学研究的再生植株都被发现是单倍体。在 14 个秋水仙素处理的植株中，有 12 个产生了能结实的小花。我们的程序的总体效率被认为高于 Laurie 和 Bennett（1988）的报告。
