Cambridge Laboratory, John Innes Centre for Plant Science Research, Colney Lane, NR4 7UJ, Norwich, UK.
Theor Appl Genet. 1994 Nov;89(6):735-41. doi: 10.1007/BF00223713.
F1 plants between two intervarietal chromosome substitution lines of European spring wheat varieties, 'Sicco' ('Chinese Spring 5B') and 'Highbury' ('Chinese Spring 5B'), were used to produce 114 doubled haploid lines, 45 by the Hordeum bulbosum technique and 69 by anther culture. These two sets of lines were characterized for variation at a range of morphological, isozyme and RFLP marker loci, and genetic maps were developed with emphasis on chromosomes 6B, 7A, 7B and 7D. A subset of lines, scored for production traits in field trials in 1986 and 1987, were analysed for quantitative trait loci (QTL). The performance of the lines for the quantitative traits studied showed no overall differences due to the method of production of the lines. QTL were located on the linkage map for ear emergence time, height, tiller weight, yield and 50-grain weight using four analytical methods. Many of these effects showed genotype x year interaction.
利用两个欧洲春小麦品种“西科”（“中国春 5B”）和“海布里”（“中国春 5B”）的种间染色体代换系之间的 F1 植株，产生了 114 条加倍单倍体系，其中 45 条通过黑麦法产生，69 条通过花药培养产生。这两组系在一系列形态、同工酶和 RFLP 标记基因座方面的变异特征进行了描述，并开发了遗传图谱，重点是 6B、7A、7B 和 7D 染色体。在 1986 年和 1987 年的田间试验中，对一组系进行了生产性状的评分，并对数量性状基因座（QTL）进行了分析。所研究的数量性状的系的表现没有因系的产生方法而产生总体差异。使用四种分析方法，在连锁图谱上定位了穗出现时间、高度、分蘖重、产量和 50 粒重的 QTL。其中许多这些效应表现出基因型 x 年份的互作。
