Cereal Research Institute, P.O. Box 391, H-6701, Szeged, Hungary.
Plant Cell Rep. 1993 Jan;12(3):175-9. doi: 10.1007/BF00239101.
Regenerable embryogenic cell suspensions initiated from immature embryo-derived friable, fast growing, embryogenic calli of GK Ságvári winter wheat (Triticum aestivum L.) served as sources of protoplasts, which were cultured in different liquid or agarose-solidified media. Protocallus formation was best on KM8p (Kao and Michayluk 1975) and GM (Li and Murai 1990) media, and protocallus growth on MS (Murashige and Skoog 1962) callus growing medium. Green shoot/plant regeneration occurred on MS regenerating medium, and rooting on MS or N6M (Mórocz et al. 1990) hormone-free media. Protocalli maintained their morphogenic capacity over 4 months, and with multiple subcultures on half-strength MS regenerating medium, the total number of regenerants could be increased. Approximately 1000 shoots/plants were regenerated and over 500 plants were transplanted in the greenhouse. The majority of them had an abnormal chromosome number and low viability, however, one plant grew to maturity and set seed.
从 GK Ságvári 冬小麦（Triticum aestivum L.）未成熟胚衍生的脆弱、快速生长、胚性愈伤组织中再生的可再生成胚细胞悬浮液可作为原生质体的来源，这些原生质体在不同的液体或琼脂糖固化培养基中进行培养。在 KM8p（Kao 和 Michayluk 1975）和 GM（Li 和 Murai 1990）培养基上原生质体原形成最好，在 MS（Murashige 和 Skoog 1962）愈伤组织生长培养基上原生质体原生长最好。绿芽/植物再生发生在 MS 再生培养基上，生根发生在 MS 或 N6M（Mórocz 等人，1990）无激素培养基上。原生质体保持其形态发生能力超过 4 个月，并在半强度 MS 再生培养基上进行多次继代培养，可增加再生体的总数。大约 1000 个芽/植物被再生，超过 500 个植物被移植到温室中。它们中的大多数具有异常的染色体数和低存活率，然而，有一株植物生长成熟并结出种子。
