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一种用于估算肝组织切片中巯基或二硫键以及总蛋白的新型联合定量细胞光度测定法;应用于长期接触镉的大鼠。

A new combined quantitative cytophotometric method for estimating sulphydryl groups or disulphide bonds, and total protein in liver sections; applied to rats chronically exposed to cadmium.

作者信息

Morselt A F, Peereboom-Stegeman J H, Jongstra-Spaapen E J

出版信息

Acta Histochem Suppl. 1986;32:221-3.

PMID:2422688
Abstract

A detailed procedure is described for a new combined quantitative cytophotometric method for estimating sulphydryl groups or (and) disulphide bonds as well as total protein. When applied to livers of cadmium treated rats and appropriate controls a dose response effect was demonstrated for the quantity disulphide bonds per unit protein.

摘要

本文描述了一种新的联合定量细胞光度法的详细步骤,该方法用于估算巯基或(和)二硫键以及总蛋白。将该方法应用于镉处理大鼠的肝脏及适当对照时,发现单位蛋白中二硫键的量呈现剂量反应效应。

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