Ma Pei, Zhang Xin-Yao, Xu Li-Jia, Wang Zhe, Xiao Pei-Gen
Chinese Academy of Medical Sciences & Peking Union Medical College, Institute of Medicinal Plant Development, Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China.
Zhongguo Zhong Yao Za Zhi. 2013 Aug;38(15):2434-7.
To study the relationship between ultra-performance liquid chromatography (UPLC) fingerprints of Rheum species and their anti-HIV 1 activities.
Twenty two samples of 16 species belonging to genus Rheum from various sources were collected and analyzed in this study. Firstly they were assayed for the HIV-1 reverse transcriptase (RT) associated ribonuclease H (RNase H) activity. Secondly the fingerprints were established by an optimized UPLC method. Sample was analyzed by UPLC-TOF-MS/MS to identify major peaks. The possible relationship between UPLC fingerprints and anti-HIV 1 activities of Rheum species were deduced by mathematical statistics method.
Samples of R. austral, R. austral, R. hotaoense exhibited good anti-HIV 1 activities with IC50 < or = 0.2 mg x L(-1). The correlation of anti-HIV 1 activities and fingerprints showed that three compounds were the main bioactive components, and their retention times were 4.74, 7.99, 21.18 min, respectively.
Compounds in Rheum species with possible anti-HIV 1 activities were deduced by spectrum-effect relationship study. This study supported for study of medicinal plants in Rheum.
研究大黄属植物的超高效液相色谱（UPLC）指纹图谱与其抗HIV-1活性之间的关系。
本研究收集并分析了来自不同来源的大黄属16个物种的22个样品。首先检测它们对HIV-1逆转录酶（RT）相关核糖核酸酶H（RNase H）的活性。其次，通过优化的UPLC方法建立指纹图谱。采用UPLC-TOF-MS/MS对样品进行分析以鉴定主要峰。通过数学统计方法推断大黄属植物UPLC指纹图谱与抗HIV-1活性之间的可能关系。
掌叶大黄、唐古特大黄、河套大黄样品表现出良好的抗HIV-1活性，IC50≤0.2mg·L-1。抗HIV-1活性与指纹图谱的相关性表明，三种化合物是主要的生物活性成分，其保留时间分别为4.74、7.99、21.18分钟。
通过谱效关系研究推断出大黄属植物中可能具有抗HIV-1活性的化合物。本研究为大黄属药用植物的研究提供了支持。
