Wirtz R A, Burkot T R, Andre R G, Rosenberg R, Collins W E, Roberts D R
Am J Trop Med Hyg. 1985 Nov;34(6):1048-54. doi: 10.4269/ajtmh.1985.34.1048.
A sandwich enzyme-linked immunosorbent assay (ELISA) for identifying Plasmodium vivax sporozoites in mosquitoes is described. Monoclonal antibodies produced against Thailand P. vivax sporozoites were used in an ELISA to detect and identify homologous sporozoites of Southeast Asian, Mexican and North Korean origin in extracts of frozen or dried infected mosquitoes. The assay was sensitive enough to detect 1 infected mosquito in a pool of 20 insects or 125-250 sporozoites per 30 microliter of mosquito extract. The use of a nonionic detergent and a single freeze-thaw to disrupt the circumsporozoite antigen significantly increased the sensitivity of the method.
本文描述了一种用于鉴定蚊子体内间日疟原虫子孢子的夹心酶联免疫吸附测定（ELISA）方法。针对泰国间日疟原虫子孢子产生的单克隆抗体被用于ELISA，以检测和鉴定来自东南亚、墨西哥和朝鲜的冷冻或干燥感染蚊子提取物中的同源子孢子。该测定法灵敏度足够高，能够在20只昆虫的混合样本中检测到1只感染蚊子，或在每30微升蚊子提取物中检测到125 - 250个子孢子。使用非离子去污剂和单次冻融来破坏环子孢子抗原显著提高了该方法的灵敏度。
