Lee M, Harrison B A, Lewis G E
U.S. Army Medical Research Unit, Institute for Medical Research, Kuala Lumpur, Malaysia.
Am J Trop Med Hyg. 1990 Apr;42(4):314-9. doi: 10.4269/ajtmh.1990.42.314.
A modified version of the standard 2-site sporozoite enzyme-linked immunosorbent assay (ELISA) using 3,3',5,5'-tetramethylbenzidine (TMB) as the substrate chromogen solution was adapted for rapid detection and identification of Plasmodium falciparum and P. vivax circumsporozoite (CS) proteins. The TMB-ELISA was evaluated using sporozoites from experimentally infected mosquitoes and laboratory colonized uninfected mosquitoes. Our data indicate comparable sensitivity levels between the TMB-ELISA and the standard ELISA, i.e., 50 P. falciparum or P. vivax sporozoites/50 microliters of test solution. Reactions inherent to the method were specific and background reactivity was minimal. The TMB-ELISA is rapid (1 hr), simple, uses a minimal amount of monoclonal antibodies, and is suitable for use in a wide range of laboratories.
一种改良版的标准双位点子孢子酶联免疫吸附测定（ELISA），使用3,3',5,5'-四甲基联苯胺（TMB）作为底物显色溶液，被用于快速检测和鉴定恶性疟原虫和间日疟原虫环子孢子（CS）蛋白。使用来自实验感染蚊子和实验室饲养的未感染蚊子的子孢子对TMB-ELISA进行了评估。我们的数据表明TMB-ELISA与标准ELISA之间的灵敏度水平相当，即每50微升测试溶液中有50个恶性疟原虫或间日疟原虫子孢子。该方法固有的反应具有特异性，背景反应性最小。TMB-ELISA快速（1小时）、简单，使用少量单克隆抗体，适用于广泛的实验室。
