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通过双抗体酶联免疫吸附测定法鉴定感染恶性疟原虫的蚊子。

Identification of Plasmodium falciparum-infected mosquitoes by a double antibody enzyme-linked immunosorbent assay.

作者信息

Burkot T R, Williams J L, Schneider I

出版信息

Am J Trop Med Hyg. 1984 Sep;33(5):783-8. doi: 10.4269/ajtmh.1984.33.783.

DOI:10.4269/ajtmh.1984.33.783
PMID:6385740
Abstract

A double antibody micro enzyme-linked immunosorbent assay (ELISA) for identifying Plasmodium falciparum sporozoites in mosquitoes is described. Using monoclonal antibodies made against South American P. falciparum sporozoites, the ELISA was able to detect and identify sporozoite antigens of South American and Asian origins in extracts of dried infected mosquitoes.

摘要

本文描述了一种用于鉴定蚊子体内恶性疟原虫子孢子的双抗体微量酶联免疫吸附测定（ELISA）方法。利用针对南美恶性疟原虫子孢子制备的单克隆抗体，该ELISA能够在干燥的感染蚊子提取物中检测和鉴定南美和亚洲来源的子孢子抗原。

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