Carrié M L, Térouanne B, Brochu M, Nicolas J C, Crastes de Paulet A
Anal Biochem. 1986 Apr;154(1):126-31. doi: 10.1016/0003-2697(86)90505-1.
Three bioluminescent immunoassay procedures for the determination of serum progesterone are compared. The sensitivity of the three procedures is similar to the sensitivity of radioimmunoassay (10 pg per tube). The use of a bioluminescent immunosorbent is particularly attractive since it has the simpler protocol. No separation step is required to remove the excess of free label (glucose-6-phosphate dehydrogenase coupled to progesterone). The free enzyme activity is not determined since the bioluminescent reaction occurs in the immunosorbent. The intensity of the signal is greater than with other procedures, and biological components contained in ethyl ether extract do not affect the quality of the assay. A good correlation with a radioimmunoassay using tritiated progesterone is obtained (r2 = 0.96) and coefficients of variation of bioluminescent assays are similar (4 to 12%) to those obtained with commercial radioimmunoassays.
比较了三种用于测定血清孕酮的生物发光免疫分析方法。这三种方法的灵敏度与放射免疫分析的灵敏度相似（每管10皮克）。使用生物发光免疫吸附剂特别有吸引力，因为它的操作流程更简单。无需分离步骤来去除过量的游离标记物（与孕酮偶联的葡萄糖-6-磷酸脱氢酶）。由于生物发光反应在免疫吸附剂中发生，因此无需测定游离酶活性。该信号强度大于其他方法，并且乙醚提取物中含有的生物成分不会影响测定质量。与使用氚化孕酮的放射免疫分析具有良好的相关性（r2 = 0.96），并且生物发光分析的变异系数与商业放射免疫分析获得的变异系数相似（4%至12%）。
