Póvoa G, Isaksson M, Jörnvall H, Hall K
Biochem Biophys Res Commun. 1985 May 16;128(3):1071-8. doi: 10.1016/0006-291x(85)91049-6.
Serum-free medium conditioned by the human hepatoma cell line HEP G2 was shown to contain a somatomedin-binding protein with a relative molecular mass of about 35,000. This binding protein was purified to homogeneity by the use of immunoaffinity chromatography and subsequent size exclusion chromatography. Antibodies for the immunoaffinity step were raised in rabbits against a previously isolated human amniotic fluid somatomedin-binding protein. The total composition and N-terminal amino acid sequence showed the protein to be identical to the binding protein from human amniotic fluid. Both have the N-terminal structure Ala-Pro-Trp-Gln-. The HEP G2 cell line offers a useful model to study the regulation of the synthesis and secretion of human somatomedin-binding proteins.
人肝癌细胞系HEP G2条件培养液中含有一种相对分子质量约为35,000的生长调节素结合蛋白。通过免疫亲和层析及随后的尺寸排阻层析，该结合蛋白被纯化至同质。免疫亲和步骤所用的抗体是用先前分离的人羊水生长调节素结合蛋白免疫家兔制备的。总组成和N端氨基酸序列显示该蛋白与人羊水结合蛋白相同。两者均具有N端结构Ala-Pro-Trp-Gln-。HEP G2细胞系为研究人生长调节素结合蛋白的合成和分泌调节提供了一个有用的模型。
