通过亲和层析法纯化正常人尿N-乙酰-β-己糖胺酶A
Purification of normal human urinary N-acetyl-beta-hexosaminidase A by affinity chromatography.
作者信息
Banerjee D K, Basu D
出版信息
Biochem J. 1975 Jan;145(1):113-8. doi: 10.1042/bj1450113.
Abstract
N-Acetyl-beta-hexosaminidase A was purified 1000-fold from human urine by chromatography on DEAE-Sephadex followed by concanavalin A--Sepharose affinity chromatography. The optimal pH range was 4.4--4.5 for both the N-acetylglucosamine and N-acetylgalactosamine derivatives. The Km values were 0.51 mM and 0.28 mM respectively for the N-acetylglucosamine and N-acetylgalactosamine derivatives. The glycoprotein nature of the urinary enzyme was established by its affinity towards concanavalin A as well as by the presence of sialic acid, galactose, glucose, mannose and hexosamines in the molecule.
摘要
通过在DEAE-葡聚糖凝胶上进行色谱分离，随后进行伴刀豆球蛋白A-琼脂糖亲和色谱，从人尿中纯化出1000倍的N-乙酰-β-己糖胺酶A。N-乙酰葡糖胺和N-乙酰半乳糖胺衍生物的最佳pH范围均为4.4 - 4.5。N-乙酰葡糖胺和N-乙酰半乳糖胺衍生物的Km值分别为0.51 mM和0.28 mM。尿酶的糖蛋白性质通过其对伴刀豆球蛋白A的亲和力以及分子中存在的唾液酸、半乳糖、葡萄糖、甘露糖和己糖胺得以确定。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Photometric determination of sialic acids in serum and cerebrospinal fluid with the thiobarbituric acid method.用硫代巴比妥酸法光度测定血清和脑脊液中的唾液酸。
Clin Chim Acta. 1962 Jul;7:467-75. doi: 10.1016/0009-8981(62)90086-4.
3
PROTEIN-CARBOHYDRATE INTERACTION. II. INHIBITION STUDIES ON THE INTERACTION OF CONCANAVALIN A WITH POLYSACCHARIDES.蛋白质-碳水化合物相互作用。II. 伴刀豆球蛋白A与多糖相互作用的抑制研究。
Biochemistry. 1965 May;4:876-83. doi: 10.1021/bi00881a013.
4
Purification of beta-N-acetylglucosaminidase from the pig epididymis.从猪附睾中纯化β-N-乙酰氨基葡萄糖苷酶。
Biochem J. 1960 Oct;77(1):170-5. doi: 10.1042/bj0770170.
5
The thiobarbituric acid assay of sialic acids.唾液酸的硫代巴比妥酸测定法。
J Biol Chem. 1959 Aug;234(8):1971-5.
6
The assay of arylsulphatases A and B in human urine.人尿中芳基硫酸酯酶A和B的测定。
Clin Chim Acta. 1959 May;4(3):453-5. doi: 10.1016/0009-8981(59)90119-6.
7
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