Hull D S, Green K, Berdecia R
Curr Eye Res. 1986 Apr;5(4):321-4. doi: 10.3109/02713688609020058.
Rabbit corneas were mounted in water jacketed chambers and the endothelial surface perfused with either BSS (Balanced Salt Solution) or BSS-Plus for 3 hr. Unidirectional and net fluxes of sodium were similar in both groups of corneas. Bicarbonate fluxes in BSS-Plus were statistically similar to those in Krebs-Ringer solution. Bicarbonate fluxes could not be determined with BSS because the solution does not contain bicarbonate. In addition, there was no statistically significant alteration of inulin or dextran permeability when comparing perfusion with BSS and BSS-Plus. From this study it appears that BSS and BSS-Plus are comparable in their ability to maintain corneal endothelial physiologic function during in-vitro perfusion. This is in contrast to previous work which showed that BSS-Plus induced less endothelial morphologic change than BSS. It is concluded that morphologic alterations may be more sensitive parameters of endothelial stress than are fluxes and permeabilities.
将兔角膜安装在带水套的腔室中，用平衡盐溶液（BSS）或加样BSS灌注内皮表面3小时。两组角膜中钠的单向通量和净通量相似。加样BSS中的碳酸氢盐通量在统计学上与 Krebs-Ringer 溶液中的相似。用BSS无法测定碳酸氢盐通量，因为该溶液不含碳酸氢盐。此外，比较用BSS和加样BSS灌注时，菊粉或右旋糖酐的通透性没有统计学上的显著变化。从这项研究看来，在体外灌注期间，BSS和加样BSS在维持角膜内皮生理功能的能力方面具有可比性。这与之前的研究结果相反，之前的研究表明加样BSS比BSS引起的内皮形态学变化更少。得出的结论是，形态学改变可能是比通量和通透性更敏感的内皮应激参数。
