利用时间分辨荧光显微镜观察单细胞中的脂质-受体相互作用。

Visualization of lipid-receptor interactions on single cells by time-resolved imaging fluorescence microscopy.

机构信息

Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, P.O. Box 2841, D-37018, Göttingen, Germany.

出版信息

J Fluoresc. 1994 Dec;4(4):295-8. doi: 10.1007/BF01881443.

DOI:10.1007/BF01881443

PMID:24233602

Abstract

The physical interaction between plasma-membrane lipids and the epidermal growth factor (EGF)-receptor was investigated on single A431 human epidermoid carcinoma cells by monitoring fluorescence resonance energy transfer (FRET) between exogeneously added fluorescein-EGF (donor) and 2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphocholine (Bodipy-PC, acceptor) using donor-photobleaching FRET-microscopy. The measured mean FRET-efficiency of 13% is indicative of such a physical interaction and exemplifies the great potential and sensitivity of time-resolved imaging fluorescence microscopy techniques for the study of lipid-receptor interactions on single cells.

摘要

通过监测荧光共振能量转移（FRET），研究了等离子体膜脂质与表皮生长因子（EGF）受体之间的物理相互作用。在单个 A431 人表皮样癌细胞中，使用外源添加的荧光素-EGF（供体）和 2-（4,4-二氟-5,7-二甲基-4-硼-3a,4a-二氮杂-s-茚并-3-戊酰基）-1-十六酰基-sn-甘油-3-磷酸胆碱（Bodipy-PC，受体）之间的 FRET 显微镜来监测荧光共振能量转移（FRET）。测量的平均 FRET 效率为 13%，表明存在这种物理相互作用，并说明了时间分辨成像荧光显微镜技术在研究单个细胞上的脂质-受体相互作用方面的巨大潜力和敏感性。