Mattiasson B
J Immunol Methods. 1980;35(1-2):137-46. doi: 10.1016/0022-1759(80)90158-1.
A new approach to radioimmunoassay is described. Thanks to the use of an aqueous two-phase system for separation of bound and free ligand, a convenient procedure has been developed where soluble antibodies may be used and separate washing steps are unnecessary. Assay of digoxin in serum using the Partition Affinity Ligand Assay (PALA), gave results which correlated closely (r = 0.979) with those obtained with conventional RIA-procedures.
本文描述了一种放射免疫分析的新方法。由于使用了双水相系统来分离结合配体和游离配体，已开发出一种便捷的方法，该方法可使用可溶性抗体且无需单独的洗涤步骤。使用分配亲和配体分析（PALA）对血清中的地高辛进行检测，所得结果与传统放射免疫分析程序的结果密切相关（r = 0.979）。
