Istituto Superiore di Sanità, Viale Regina Elena 299, 1-00161, Rome, Italy.
Environ Sci Pollut Res Int. 1994 Dec;1(4):205-8. doi: 10.1007/BF02986531.
The separation and determination of sub-μmol/l levels of six environmentally significant As compounds was accomplished by means of high performance liquid chromatography (HPLC) combined online with inductively coupled plasma mass spectrometry (ICP-MS). The species of interest (arsenite and arsenate, monomethylarsonic acid, dimethylarsinic acid, arsenobetaine and arsenocholine) were quantified in fish and mussel extracts after separation on a Dionex AS7 column equipped with an on-guard AG7 column using a bicarbonate buffer as the mobile phase in a gradient mode. The species thus eluted were directly forwarded to the ICP-MS detector. The detection power of the overall system allows each As form to be determined at concentrations as low a 0.0013-0.0027 μmol 1(-1).
采用高效液相色谱（HPLC）与电感耦合等离子体质谱（ICP-MS）在线联用的方法，实现了对六种环境意义重大的砷化合物的亚微摩尔级别的分离和测定。在 Dionex AS7 柱上分离后，使用碳酸氢盐缓冲液作为流动相，在梯度模式下，在带有Guard AG7 柱的设备上，对鱼和贻贝类提取物中的感兴趣的物质（亚砷酸盐和砷酸盐、一甲基砷酸、二甲基砷酸、砷甜菜碱和砷胆碱）进行定量。洗脱的物质直接传输到 ICP-MS 检测器。整个系统的检测能力使得每种砷形态都可以在 0.0013-0.0027 μmol 1(-1)的低浓度下进行测定。
