West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China; Department of Clinical Laboratory, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610041, China.
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
Food Chem. 2020 Oct 15;327:127033. doi: 10.1016/j.foodchem.2020.127033. Epub 2020 May 19.
Some varieties of edible mushrooms can accumulate high contents of arsenic, which is a public health concern. In this study, we developed a high-performance liquid chromatography method linked to inductively coupled plasma triple quadrupole mass spectrometry (HPLC-ICP-MS/MS) for sensitive and accurate determination of arsenite, arsenate, monomethylarsonic acid, dimethylarsinic acid, arsenocholine and arsenobetaine in edible mushrooms. The six arsenic species were extracted ultrasonically from mushrooms using ultrapure water at 60 °C as the solvent, separated on a PRP-X100 anion exchange column (4.1 × 250 mm, 10 µm), with 20 mmol/L NHHCO and 50 mmol/L (NH)CO as the mobile phase, and quantified using ICP-MS/MS in the oxygen reaction mode. The linear range of the method was 0.5 µg/L-100 µg/L with detection and quantification limits of 2.5 µg/kg-10 µg/kg (S/N = 3), and 8 µg/kg-33 µg/kg (S/N = 10), respectively. This method was applied successfully to the detection and speciation of arsenic in eight varieties (266 samples) of mushrooms. Our results indicated that most wild edible mushrooms contained organic arsenic, mainly arsenobetaine and arsenocholine. However, the inorganic arsenic content of Armillariella tabescens (3.63 mg/kg) and parts of the cultivated Agaricus blazei murrill (up to 4.50 mg/kg) were relatively high, which is potential risk to the health of consumers.
一些可食用蘑菇品种可积累高含量的砷，这是一个公共卫生关注点。在本研究中，我们开发了一种高效液相色谱法与电感耦合等离子体质谱三重四极杆联用（HPLC-ICP-MS/MS），用于灵敏准确地测定食用蘑菇中的亚砷酸盐、砷酸盐、一甲基砷酸、二甲基砷酸、砷胆碱和砷甜菜碱。这六种砷形态通过超声提取，使用 60°C 的超纯水作为溶剂，在 PRP-X100 阴离子交换柱（4.1×250mm，10μm）上分离，以 20mmol/L NH₄HCO₃和 50mmol/L (NH₄)₂CO₃作为流动相，使用氧反应模式的 ICP-MS/MS 定量。该方法的线性范围为 0.5μg/L-100μg/L，检测限和定量限分别为 2.5μg/kg-10μg/kg（S/N=3）和 8μg/kg-33μg/kg（S/N=10）。该方法成功应用于 8 种（266 个样本）蘑菇中砷的检测和形态分析。结果表明，大多数野生食用蘑菇含有有机砷，主要为砷甜菜碱和砷胆碱。然而，阿魏蘑（3.63mg/kg）和部分栽培双孢蘑菇（高达 4.50mg/kg）的无机砷含量相对较高，这对消费者的健康构成潜在风险。
