Higgins S J, Smith S E, Wilson J
Mol Cell Endocrinol. 1982 Jun;27(1):55-65. doi: 10.1016/0303-7207(82)90062-4.
Functional development of the ventral prostate and seminal vesicles in the male rat has been examined by incubating tissue with [35S] methionine and using specific antisera to detect the synthesis of individual marker proteins. 4 major secretory proteins of the seminal vesicles were chosen along with 4 major prostatic secretory peptides, including 3 which form the prostatic steroid-binding protein. None of the seminal vesicle proteins was synthesised before 25-30 days but all were then induced in parallel to reach adult values by 45-50 days. The 3 peptides of the prostatic steroid-binding protein were synthesised at the earliest age studied (10 days) and were also induced in parallel to maximal levels by 25-30 days. In contrast, the 4th prostatic marker, a glycopeptide, was not synthesised until about 25 days. Treatment of male rats from 3 days of age with testosterone induced precocious development of the seminal vesicles, causing substantial synthesis of marker proteins well before their synthesis would normally have been expected, and also advanced the synthesis of the prostatic 22 K glycopeptide. The results confirm that functional development in the ventral prostate precedes that of the seminal vesicles but that within the ventral prostate there is a considerable difference in the developmental profiles of its secretory proteins.
通过将组织与[35S]甲硫氨酸一起孵育，并使用特异性抗血清来检测单个标记蛋白的合成，对雄性大鼠腹侧前列腺和精囊的功能发育进行了研究。选择了精囊的4种主要分泌蛋白以及4种主要的前列腺分泌肽，其中包括3种构成前列腺类固醇结合蛋白的肽。在25 - 30天之前，精囊蛋白均未合成，但之后所有蛋白均被平行诱导，到45 - 50天时达到成年水平。在研究的最早年龄（10天）就合成了前列腺类固醇结合蛋白的3种肽，并且也被平行诱导，到25 - 30天时达到最大水平。相比之下，第4种前列腺标记物，一种糖肽，直到约25天时才合成。从3日龄开始用睾酮处理雄性大鼠，可诱导精囊早熟发育，导致标记蛋白在正常预期合成时间之前大量合成，并且还提前了前列腺22K糖肽的合成。结果证实，腹侧前列腺的功能发育先于精囊，但在腹侧前列腺内，其分泌蛋白的发育模式存在相当大的差异。
