Oda Y, Hirabayashi J, Kasai K
J Biochem. 1986 Apr;99(4):1063-70. doi: 10.1093/oxfordjournals.jbchem.a135570.
Monoclonal antibodies against an endogenous beta-galactoside-binding lectin (monomer molecular weight 14,000, 14K lectin) of chick embryo were prepared and characterized. The inhibitory activities against hemagglutination, antigenic determinants and binding specificities were examined. Monoclonal antibody S1A4-5 strongly inhibited the hemagglutination activity of this lectin. This antibody did not bind to any cyanogen bromide (BrCN) fragment of the lectin. Another monoclonal antibody, S1A4-3, bound to one of the BrCN fragments (residues 34-66). However, this antibody inhibited hemagglutination only weakly. The bindings to isolectins of beta-galactoside-binding lectin, namely 14K lectin (monomer molecular weight 16,000) and a third species which is assumed to be a hybrid molecule consisting of 14K and 16K lectin subunits, were examined. The antibody SIA4-5 bound to 14K lectin but not to 16K lectin. In the case of the third species, intermediate binding was observed.
制备并鉴定了针对鸡胚内源性β-半乳糖苷结合凝集素（单体分子量14,000，14K凝集素）的单克隆抗体。检测了其对血凝、抗原决定簇和结合特异性的抑制活性。单克隆抗体S1A4 - 5强烈抑制该凝集素的血凝活性。该抗体不与凝集素的任何溴化氰（BrCN）片段结合。另一种单克隆抗体S1A4 - 3与其中一个BrCN片段（第34 - 66位氨基酸残基）结合。然而，该抗体仅微弱抑制血凝。检测了其与β-半乳糖苷结合凝集素的同工凝集素的结合情况，即14K凝集素（单体分子量16,000）和假定为由14K和16K凝集素亚基组成的杂种分子的第三种同工凝集素。抗体SIA4 - 5与14K凝集素结合，但不与16K凝集素结合。对于第三种同工凝集素，观察到中等程度的结合。
