文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Joubert-Caron R, Caron M, Bochet P, Chadli A, Delaporte P, Schuller E, Bladier D

Laboratoire de Biochimie et Technologie des Protéines, Université Paris-Nord, Bobigny, France.

Int J Biochem. 1994 Jun;26(6):813-23. doi: 10.1016/0020-711x(94)90111-2.

An antiserum raised against a 14 kDa beta-galactoside specific lectin from human brain (HBL14) was used to probe blots from samples of serum and cerebrospinal fluid. The only HBL14-immunoreactive material detected was heavy and light chains of a beta-galactoside-binding IgG fraction (lectin-like IgG). 2. Lectin-like IgG, as well as IgG Fab fragments, compete with HBL14 for binding either to anti-HBL14 antibody or to a lactosyl polyacrylamide-based copolymer. 3. Purification of lectin-like IgG was obtained by affinity chromatography on immobilized rabbit anti-lectin immunoglobulins. The carbohydrate-binding specificity of the purified molecules was restricted to beta-Gal-containing structures and close to the HBL14 one.

用针对人脑中一种14 kDaβ-半乳糖苷特异性凝集素（HBL14）制备的抗血清检测血清和脑脊液样本的印迹。检测到的唯一HBL14免疫反应性物质是β-半乳糖苷结合IgG组分（凝集素样IgG）的重链和轻链。2. 凝集素样IgG以及IgG Fab片段与HBL14竞争结合抗HBL14抗体或基于乳糖基聚丙烯酰胺的共聚物。3. 通过固定化兔抗凝集素免疫球蛋白的亲和层析纯化凝集素样IgG。纯化分子的碳水化合物结合特异性限于含β-半乳糖的结构，且与HBL14相近。

Joubert-Caron R, Caron M, Bochet P, Chadli A, Delaporte P, Schuller E, Bladier D

Laboratoire de Biochimie et Technologie des Protéines, Université Paris-Nord, Bobigny, France.

Int J Biochem. 1994 Jun;26(6):813-23. doi: 10.1016/0020-711x(94)90111-2.

An antiserum raised against a 14 kDa beta-galactoside specific lectin from human brain (HBL14) was used to probe blots from samples of serum and cerebrospinal fluid. The only HBL14-immunoreactive material detected was heavy and light chains of a beta-galactoside-binding IgG fraction (lectin-like IgG). 2. Lectin-like IgG, as well as IgG Fab fragments, compete with HBL14 for binding either to anti-HBL14 antibody or to a lactosyl polyacrylamide-based copolymer. 3. Purification of lectin-like IgG was obtained by affinity chromatography on immobilized rabbit anti-lectin immunoglobulins. The carbohydrate-binding specificity of the purified molecules was restricted to beta-Gal-containing structures and close to the HBL14 one.

用针对人脑中一种14 kDaβ-半乳糖苷特异性凝集素（HBL14）制备的抗血清检测血清和脑脊液样本的印迹。检测到的唯一HBL14免疫反应性物质是β-半乳糖苷结合IgG组分（凝集素样IgG）的重链和轻链。2. 凝集素样IgG以及IgG Fab片段与HBL14竞争结合抗HBL14抗体或基于乳糖基聚丙烯酰胺的共聚物。3. 通过固定化兔抗凝集素免疫球蛋白的亲和层析纯化凝集素样IgG。纯化分子的碳水化合物结合特异性限于含β-半乳糖的结构，且与HBL14相近。