子痫前期影响脐血 NK 细胞激活受体的表达和血清细胞因子水平，但不影响 CB 单核细胞的特征。
Pre-eclampsia affects cord blood NK cell expression of activation receptors and serum cytokine levels but not CB monocyte characteristics.
机构信息
Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.
出版信息
Am J Reprod Immunol. 2014 Feb;71(2):178-88. doi: 10.1111/aji.12169. Epub 2013 Nov 14.
PROBLEM
Maternal immunopathology in pre-eclampsia is well studied; however, less is known regarding the immunological effects on the newborns. Increased inflammation and activation of immune cells at the fetal-maternal interface in pre-eclampsia could influence the neonatal immune compartment.
METHOD OF STUDY
Monocytes and natural killer (NK) cells from cord blood (CB) of children with pre-eclamptic or healthy mothers were analyzed by flow cytometry for surface markers and intracellular cytokines. In addition, serum cytokine profiles were investigated using ELISA or cytometric bead array.
RESULTS
Neonates born to pre-eclamptic mothers had an inflammatory serum cytokine profile. While CB monocyte characteristics seemed unaffected, CB NK cells from pre-eclamptic pregnancies had higher NKp30, but borderline lower NKG2D expression.
CONCLUSION
In utero inflammatory priming of neonatal innate immunity taking place in pre-eclamptic pregnancies might influence specific NK cell functions in newborns.
问题
子痫前期的母体免疫病理学研究较为充分；然而，对于其对子代新生儿的免疫学影响知之甚少。子痫前期时胎儿-母体界面的炎症反应增加和免疫细胞的激活可能会影响新生儿的免疫区室。
方法
通过流式细胞术分析来自子痫前期或健康产妇脐带血中的单核细胞和自然杀伤 (NK) 细胞的表面标志物和细胞内细胞因子。此外，还使用 ELISA 或流式细胞术检测细胞因子分析来研究血清细胞因子谱。
结果
来自子痫前期母亲的新生儿具有炎症性血清细胞因子谱。虽然 CB 单核细胞特征似乎未受影响，但来自子痫前期妊娠的 CBNK 细胞具有更高的 NKp30 表达，但 NKG2D 表达有边界降低。
结论
在子痫前期妊娠中，胎儿期固有免疫的宫内炎症预刺激可能会影响新生儿 NK 细胞的特定功能。
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