Department of Pediatric Heart Center, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China.
Chin Med J (Engl). 2013 Nov;126(22):4282-8.
Pediatric patients are susceptible to lung injury that does not respond to traditional therapies. Total liquid ventilation has been developed as an alternative ventilatory strategy for severe lung injury. The aim of this study is to investigate the effect of total liquid ventilation on oleic acid (OA)-induced lung injury in piglets.
Twelve Chinese immature piglets were induced acute lung injury by OA. Twelve piglets were randomly treated with conventional gas ventilation (control group) or total liquid ventilation (study group) for 240 minutes. Samples for blood gas analysis were collected before, and at 60-minute intervals after OA-induced lung injury. The degree of lung injury was quantified by histologic examination. The inflammatory cells and the levels of IL-1β, IL-6, IL-10 and TNF-α in plasma, tissue and bronchoalveolar lavage were analyzed.
Neutrophil and macrophage counts in bronchoalveolar lavage were significantly decreased in the study group (P < 0.05). The total lung injury score was also reduced in the study group (P < 0.05). The concentrations of IL-1β, IL-6, IL-10 and TNF-α in plasma, tissue and bronchoalveolar lavage were significantly reduced in the study group (P < 0.05).
Total liquid ventilation reduces biochemical and histologic OA-induced lung injury in piglets.
儿科患者易发生对传统疗法无反应的肺损伤。全液体通气已被开发为严重肺损伤的替代通气策略。本研究旨在探讨全液体通气对油酸（OA）诱导的仔猪肺损伤的影响。
12 只中国未成熟仔猪通过 OA 诱导急性肺损伤。12 只仔猪随机接受常规气体通气（对照组）或全液体通气（研究组）治疗 240 分钟。在 OA 诱导肺损伤前和 60 分钟间隔采集血气分析样本。通过组织学检查量化肺损伤程度。分析血浆、组织和支气管肺泡灌洗液中炎症细胞和 IL-1β、IL-6、IL-10 和 TNF-α 的水平。
研究组支气管肺泡灌洗液中的中性粒细胞和巨噬细胞计数明显减少（P < 0.05）。研究组的总肺损伤评分也降低（P < 0.05）。研究组血浆、组织和支气管肺泡灌洗液中 IL-1β、IL-6、IL-10 和 TNF-α 的浓度明显降低（P < 0.05）。
全液体通气可减轻油酸诱导的仔猪肺的生化和组织学损伤。
