Department of Chemistry, Southern Methodist University, Dallas, TX 75275, USA; Center for Drug Discovery, Design, and Delivery (CD4), Southern Methodist University, Dallas, TX 75275, USA.
J Inorg Biochem. 2014 Apr;133:136-42. doi: 10.1016/j.jinorgbio.2013.10.010. Epub 2013 Oct 21.
Hydrogen sulfide (H2S) is a biologically generated, gaseous signaling molecule that mediates a wide range of physiological functions and is misregulated in numerous pathologies ranging from neurodegenerative disease to hypertension to diabetes. Despite swelling interest, a deeper understanding of the biological roles played by H2S has been hindered by a lack of tools for the real-time visualization of its production in living organisms. Recently, reaction-based fluorescent probes have emerged as an ideal approach for selective H2S imaging and are attracting increasing attention with many new innovative designs being introduced. This review will highlight some of the most fruitful reaction-based strategies, including reduction-based, nucleophilic-based, and metal sulfide precipitation-based fluorescent sensors. Strategies to address the key design challenges of sensitivity, selectivity, in vivo compatibility, and quantification will be discussed using examples of recently developed molecular scaffolds for selective H2S detection.
硫化氢 (H2S) 是一种生物生成的气态信号分子，介导广泛的生理功能，并在从神经退行性疾病到高血压到糖尿病等多种病理状态下失调。尽管人们越来越感兴趣，但由于缺乏实时可视化其在活生物体中产生的工具，对 H2S 所发挥的生物学作用的深入了解受到了阻碍。最近，基于反应的荧光探针已成为选择性 H2S 成像的理想方法，并因其许多新的创新设计而受到越来越多的关注。本综述将重点介绍一些最有成效的基于反应的策略，包括基于还原、亲核和金属硫化物沉淀的荧光传感器。将使用最近开发的用于选择性 H2S 检测的分子支架的示例，讨论解决灵敏度、选择性、体内相容性和定量的关键设计挑战的策略。
