Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210, Vienna, Austria.
Parasitol Res. 2014 Feb;113(2):513-6. doi: 10.1007/s00436-013-3682-y. Epub 2013 Nov 16.
A novel direct PCR assay for the detection of Dirofilaria spp. from EDTA blood, Knott test, FTA cards, adult filarial worms, skin nodules and Dirofilaria spp.-infected mosquitoes was tested. Larval and adult DNA of Dirofilaria spp. from FTA cards, from the mosquito vector and from worm fragments without prior DNA extraction was successfully obtained. As little as 3.11 larvae/100 μl blood on FTA cards could be detected. Thus, direct PCR is capable of directly detecting first larval stages in the blood, third larval stages in the mosquito vector and pieces of mature stages of Dirofilaria spp. The assay is a rapid, sensitive and cost-effective alternative to standard PCR.
本研究旨在评估一种新型直接 PCR 检测方法，用于检测 EDTA 抗凝血、Knott 试验、FTA 卡、成虫丝虫、皮肤结节和感染 Dirofilaria spp.的蚊子中的 Dirofilaria spp.。从 FTA 卡、蚊媒和虫体片段中提取幼虫和成虫的 Dirofilaria spp.DNA，无需预先进行 DNA 提取。在 FTA 卡上，可成功检测到低至 3.11 条幼虫/100μl 血液。因此，直接 PCR 可直接检测血液中的第一幼虫期、蚊媒中的第三幼虫期和 Dirofilaria spp.的成虫期。该检测方法快速、敏感且经济有效，是标准 PCR 的替代方法。
