Department of Biology, Wuhan University, Wuhan, The People's Republic of China.
Plant Cell Rep. 1988 Mar;7(2):107-10. doi: 10.1007/BF00270116.
A technique named two-step osmotic shock was developed for isolating generative cells (GCs): pollen grains were incubated in 20% sucrose solution and shocked by adding water. This caused the pollen grains to burst and release their contents including GCs. By subsequent filtration and centrifugation the isolated GCs were purified. The viability of GCs before and after purification was confirmed by fluorescein diacetate (FDA) test. This procedure offers a rapid and effective means to obtain living GCs in quantities.
一种名为两步渗透冲击的技术被开发用于分离生殖细胞（GC）：花粉粒在 20%蔗糖溶液中孵育，然后通过加水冲击使其破裂并释放出包括 GC 在内的内容物。通过后续的过滤和离心，分离出的 GC 得到纯化。通过荧光素二乙酸（FDA）试验确认 GC 在纯化前后的活力。该程序提供了一种快速有效的方法，可以获得大量的活 GC。
