Welsh Plant Breeding Station, University of Wales, Plas Gogerddan, near Aberystwyth, SY23 3EB, Wales, UK.
Theor Appl Genet. 1984 Feb;67(4):367-75. doi: 10.1007/BF00272876.
Methods currently available for evaluating pollen quality in vitro include, (a) tests of germinability; (b) tests of the stainability of the vegetative cell contents; (c) tests for enzyme activity, and (d) the fluorochromatic procedure (FCR), which tests principally the integrity of the plasmalemma of the vegetative cell. Using germinability in vitro as a standard, a comparison has been made between histochemical methods of classes (b), (c) and (d) in application to various pollens, immature, mature, and treated in ways known to affect viability and membrane state. Predictably, the lowest correlation was obtained with tests of stainability. The highest was given by the FCR, which generally provided an excellent guide to potential germinability. The FCR procedure is subject to various limitations, however, (a) A high correlation between FCR and germinability can only be expected when mature, ripe pollen is used; with immature pollen, the FCR will predict excessively high potential germinability. (b) The FCR may also predict a higher potential level of pollen function than in vitro germinability when the germination medium is sub-optimal. In this situation, however, it will generally give a better guide to fertilising capacity, (c) The FCR is not a test of pollen viability. Like germinability in vitro, it can yield a negative score with pollen which is nevertheless capable of functioning. For example, false negatives will be obtained with some species if the pollen is not properly pre-conditioned by rehydration before testing, an important point in monitoring stored pollen. The paper includes a brief discussion of the rationale of pollen testing.
(a)发芽率测试；(b)营养细胞内容物染色能力测试；(c)酶活性测试；以及(d)荧光染色程序（FCR），该程序主要测试营养细胞质膜的完整性。以体外发芽率作为标准，对（b）、（c）和（d）类组织化学方法在应用于各种花粉（未成熟、成熟和经过已知会影响活力和膜状态的处理）时进行了比较。可以预见的是，与染色能力测试相比，相关性最低。FCR 给出了最高的相关性，它通常为潜在的发芽能力提供了极好的指导。然而，FCR 程序受到各种限制：(a)只有在使用成熟、成熟花粉时，FCR 与发芽率之间才能预期高度相关；对于未成熟花粉，FCR 将预测过高的潜在发芽能力。(b)当发芽培养基不是最佳时，FCR 也可能预测花粉功能的潜在水平高于体外发芽率。然而，在这种情况下，它通常会更好地指导受精能力。(c)FCR 不是花粉活力的测试。与体外发芽率一样，它可以对仍然能够发挥作用的花粉产生阴性评分。例如，如果在测试前不对花粉进行适当的水合预处理，一些物种将出现假阴性，这在监测储存花粉时是一个重要的问题。本文还简要讨论了花粉测试的基本原理。
