Department of Genetics, Agriculturai University, Generaal Foulkesweg 53, NL-6703 BW, Wageningen, The Netherlands.
Plant Cell Rep. 1988 May;7(3):193-6. doi: 10.1007/BF00269321.
The growth of cell aggregates from a rye suspension culture was tested at low density in three culture systems. Mass seeding was the most supportive system, followed by agarose droplets. Microdroplet culture using Cuprak dishes was the least effective system. Growth was stimulated by the presence of a feeder layer of suspension cells but only if the feeder contact with the nursed cells was via a liquid and not a gaseous phase. Plating efficiences were enhanced by the feeder effect, whereas the subsequent growth rates were less affected. The techniques described should prove useful in programs aimed at the in vitro genetic manipulation of rye or other cereals.
从黑麦悬浮培养物中生长的细胞聚集体在三种培养系统中进行了低密度测试。大量接种是最具支持性的系统，其次是琼脂糖液滴。使用 Cuprak 培养皿的微滴培养是最无效的系统。悬浮细胞的饲养层的存在刺激了生长，但前提是饲养细胞与被滋养细胞的接触是通过液相而不是气相。接种效率受饲养效应增强，但随后的生长速率受影响较小。所描述的技术应该对旨在体外遗传操纵黑麦或其他谷物的计划有用。
