Aasjord P, Haaheim L R
Acta Pathol Microbiol Immunol Scand C. 1985 Dec;93(6):245-50. doi: 10.1111/j.1699-0463.1985.tb02952.x.
Two monoclonal antibodies against staphylococcal lipoteichoic acid (LTA) were made by fusing P3X63Ag8 myeloma cells and splenocytes from mice immunized with purified LTA. Both were isotyped as being IgM kappa. Their specificities were determined by enzyme-linked immunosorbent assays indicating that both antibodies reacted with the glycerol-phosphate backbone, while one of them also had some affinity for the alanyl substituent. Antibodies in serum from 7 multiple sclerosis (MS) patients and serum and cerebrospinal fluid (CSF) from 7 non-MS patients apparently reacted with the sugar moiety of LTA. In contrast, CSF antibodies from 6 of the 7 MS patients and 1 of the 7 non-MS patients had affinity for the alanine residue. This non-MS patient also had serum antibodies against the alanine residue. None of the other sera tested appeared to contain such antibodies.
通过将P3X63Ag8骨髓瘤细胞与用纯化脂磷壁酸（LTA）免疫的小鼠脾细胞融合，制备了两种抗葡萄球菌脂磷壁酸的单克隆抗体。两者均被鉴定为IgM κ型。通过酶联免疫吸附测定确定它们的特异性，表明两种抗体均与甘油磷酸主链反应，而其中一种对丙氨酰取代基也有一定亲和力。7例多发性硬化症（MS）患者血清中的抗体以及7例非MS患者的血清和脑脊液（CSF）明显与LTA的糖部分发生反应。相比之下，7例MS患者中的6例和7例非MS患者中的1例的脑脊液抗体对丙氨酸残基具有亲和力。该非MS患者血清中也存在针对丙氨酸残基的抗体。其他检测的血清似乎均不含此类抗体。
