Zahr Omar K, Blum Amy Szuchmacher
Department of Chemistry, McGill University, Montreal, QC, Canada.
Methods Mol Biol. 2014;1108:105-12. doi: 10.1007/978-1-62703-751-8_8.
Bottom-up self-assembly techniques are a powerful method of building nanoscale structures in an energy efficient and cost effective manner. The use of biological templates, such as proteins, takes advantage of the monodispersity and precision of naturally evolved systems to produce highly organized assemblies of small molecules and nanoparticles. Here we describe a method whereby arginine residues on a viral coat protein (Tobacco Mosaic Virus) are targeted by bis(p-sulfonatophenyl)phenylphosphine (BSPP)-passivated gold nanoparticles with high specificity to create 22 nm rings.
自下而上的自组装技术是一种以节能且经济高效的方式构建纳米级结构的强大方法。利用生物模板，如蛋白质，借助自然进化系统的单分散性和精确性来生产小分子和纳米颗粒的高度有序组装体。在此，我们描述了一种方法，通过该方法，双（对磺酸钠苯基）苯基膦（BSPP）钝化的金纳米颗粒以高特异性靶向病毒外壳蛋白（烟草花叶病毒）上的精氨酸残基，从而形成22纳米的环。
