Brown Adam D, Culver James N
Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, MD, USA.
Methods Mol Biol. 2014;1108:113-21. doi: 10.1007/978-1-62703-751-8_9.
A modified version of the rod-shaped Tobacco Mosaic Virus (TMV1cys) provides a robust template for the self-assembly and fabrication of high-surface-area materials for numerous applications including batteries and sensors. TMV1cys surface fabrication is facilitated by the addition of a single cysteine residue to the virus coat protein that directs the vertical attachment of the virus particle onto substrate surfaces and enables deposition of functional inorganic layers. Here we describe the production and purification of the virus, its assembly onto suitable surfaces, and coating with metallic nickel and cobalt.
棒状烟草花叶病毒（TMV1cys）的一种改良版本为自组装和制造高表面积材料提供了一个强大的模板，这些材料可用于包括电池和传感器在内的众多应用。通过在病毒外壳蛋白中添加单个半胱氨酸残基来促进TMV1cys的表面制造，该残基可引导病毒颗粒垂直附着在基底表面，并能够沉积功能性无机层。在此，我们描述了病毒的生产和纯化、其在合适表面上的组装以及用金属镍和钴进行的涂层处理。
