Hussain Raja Azadar, Badshah Amin, Tahir Muhammad Nawaz, Bano Asghari
Coordination Chemistry Laboratory, Department of Chemistry, Quaid-i-Azam University, Islamabad, Pakistan.
J Biochem Mol Toxicol. 2014 Feb;28(2):60-8. doi: 10.1002/jbt.21536. Epub 2013 Nov 14.
Ferrocene-incorporated selenoureas 1-(4-methoxybenzoyl)-3-(4-ferrocenylphenyl)selenourea (P4Me), 1-(3-methoxybenzoyl)-3-(4-ferrocenylphenyl)selenourea (P3Me), and 1-(2-methoxybenzoyl)-3-(4-ferrocenylphenyl)selenourea (P2Me) were synthesized and characterized by nuclear magnetic resonance, Fourier transform infrared spectroscopy, atomic absorption spectroscopy, CHNS, and single-crystal X-ray diffraction. DNA interaction of the compounds was investigated with cyclic voltammetry, UV-visible spectroscopy, and viscometry, which is a prerequisite for anticancer agents. Drug-DNA binding constant was found to vary in the sequence: K(P4Me) (4.9000 × 10⁴ M⁻¹) > K(P2Me) (2.318 × 10⁴ M⁻¹) > K(P3Me) (1.296 × 10⁴ M⁻¹). Antioxidant (1,1-diphenyl-2-picrylhydrazyl), antifungal (against Faussarium solani and Helmentosporium sativum), and antibacterial (against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis) activities have also been reported in addition.
合成了含二茂铁的硒脲类化合物1-(4-甲氧基苯甲酰基)-3-(4-二茂铁基苯基)硒脲(P4Me)、1-(3-甲氧基苯甲酰基)-3-(4-二茂铁基苯基)硒脲(P3Me)和1-(2-甲氧基苯甲酰基)-3-(4-二茂铁基苯基)硒脲(P2Me)，并通过核磁共振、傅里叶变换红外光谱、原子吸收光谱、CHNS分析和单晶X射线衍射对其进行了表征。采用循环伏安法、紫外可见光谱法和粘度测定法研究了这些化合物与DNA的相互作用，这是抗癌药物的一个先决条件。发现药物与DNA的结合常数按以下顺序变化：K(P4Me)(4.9000×10⁴ M⁻¹)>K(P2Me)(2.318×10⁴ M⁻¹)>K(P3Me)(1.296×10⁴ M⁻¹)。此外，还报道了它们的抗氧化活性(1,1-二苯基-2-苦基肼)、抗真菌活性(针对茄丝核菌和小麦根腐病菌)以及抗菌活性(针对大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌和枯草芽孢杆菌)。
