Department of Obstetrics and Gynecology, Tongji Hospital, Tongji University, No,389 Xincun Road, Shanghai 200065, China.
Eur J Med Res. 2013 Nov 19;18(1):46. doi: 10.1186/2047-783X-18-46.
The aim of this study is to investigate the effects of polyphenol extract from Phyllanthus emblica (PEEP) on cervical cancer cells and to explore the underlying mechanism.
MTT assay was used to measure inhibition of proliferation of cervical cancer (HeLa) cells after treatment with PEEP at concentrations of 0, 50, 100, 150, and 200 mg/ml for 48 hours. HeLa cells were treated with PEEP (150 mg/ml) for 48 hours in the following analysis. Karyomorphism was assessed by immunofluorescence using DAPI staining, and cell apoptosis and cell cycle were assessed using flow cytometry. Three apoptotic marker proteins, namely, Fas, FasL, and cleaved caspase-8, were assessed by western blotting.
PEEP inhibited the growth of HeLa cells, and the optimum concentration of PEEP was 150 mg/ml. In addition, the karyomorphism of HeLa cells after treatment with PEEP was abnormal. Furthermore, PEEP induced arrest of the HeLa cell cycle at G2/M phase, and triggered apoptosis. PEEP also induced significant Fas and FasL activation, and cleavage of caspase-8.
Our study indicates that PEEP is effective in inhibiting HeLa cell proliferation by inducing cell cycle arrest at G2/M phase and inducing apoptosis.
本研究旨在探讨余甘子多酚提取物（PEEP）对宫颈癌细胞的影响，并探讨其潜在机制。
采用 MTT 法检测不同浓度（0、50、100、150 和 200mg/ml）的 PEEP 处理宫颈癌（HeLa）细胞 48 小时后对细胞增殖的抑制作用。采用 PEEP（150mg/ml）处理 HeLa 细胞 48 小时进行以下分析。用 DAPI 染色通过免疫荧光法评估核型，用流式细胞术评估细胞凋亡和细胞周期。通过 Western blot 检测三种凋亡标记蛋白 Fas、FasL 和裂解的 caspase-8。
PEEP 抑制 HeLa 细胞生长，PEEP 的最佳浓度为 150mg/ml。此外，PEEP 处理后的 HeLa 细胞的核型异常。此外，PEEP 诱导 HeLa 细胞周期停滞在 G2/M 期，并引发细胞凋亡。PEEP 还诱导 Fas 和 FasL 的显著激活，以及 caspase-8 的裂解。
我们的研究表明，PEEP 通过诱导 G2/M 期细胞周期阻滞和诱导细胞凋亡有效抑制 HeLa 细胞增殖。
