Berberidou Chrysanthi, Xanthopoulos Konstantinos, Paspaltsis Ioannis, Lourbopoulos Athanasios, Polyzoidou Eleni, Sklaviadis Theodoros, Poulios Ioannis
Laboratory of Physical Chemistry; Department of Chemistry; Aristotle University of Thessaloniki; Thessaloniki, Greece; Prion Disease Research Group; Laboratory of Pharmacology; School of Pharmacy; Aristotle University of Thessaloniki; Thessaloniki, Greece.
Prion. 2013 Nov-Dec;7(6):488-95. doi: 10.4161/pri.27180. Epub 2013 Nov 18.
Prions are notorious for their extraordinary resistance to traditional methods of decontamination, rendering their transmission a public health risk. Iatrogenic Creutzfeldt-Jakob disease (iCJD) via contaminated surgical instruments and medical devices has been verified both experimentally and clinically. Standard methods for prion inactivation by sodium hydroxide or sodium hypochlorite have failed, in some cases, to fully remove prion infectivity, while they are often impractical for routine applications. Prion accumulation in peripheral tissues and indications of human-to-human bloodborne prion transmission, highlight the need for novel, efficient, yet user-friendly methods of prion inactivation. Here we show both in vitro and in vivo that homogenous photocatalytic oxidation, mediated by the photo-Fenton reagent, has the potential to inactivate the pathological prion isoform adsorbed on metal substrates. Photocatalytic oxidation with 224 μg mL(-1) Fe (3+), 500 μg mL(-1) h(-1) H 2O 2, UV-A for 480 min lead to 100% survival in golden Syrian hamsters after intracranial implantation of stainless steel wires infected with the 263K prion strain. Interestingly, photocatalytic treatment of 263K infected titanium wires, under the same experimental conditions, prolonged the survival interval significantly, but failed to eliminate infectivity, a result that we correlate with the increased adsorption of PrP(Sc) on titanium, in comparison to stainless steel. Our findings strongly indicate that our, user--and environmentally--friendly protocol can be safely applied to the decontamination of prion infected stainless steel surfaces.
朊病毒因其对传统去污方法具有极强的抵抗力而声名狼藉，这使其传播成为公共卫生风险。通过受污染的手术器械和医疗设备传播的医源性克雅氏病（iCJD）已在实验和临床中得到证实。用氢氧化钠或次氯酸钠灭活朊病毒的标准方法在某些情况下未能完全消除朊病毒的传染性，而且它们在常规应用中往往不切实际。朊病毒在外周组织中的积累以及人与人之间血源性朊病毒传播的迹象，凸显了对新型、高效且用户友好的朊病毒灭活方法的需求。在此我们表明，在体外和体内，由光芬顿试剂介导的均相光催化氧化有潜力灭活吸附在金属底物上的病理性朊病毒异构体。用224μg mL(-1) Fe(3+)、500μg mL(-1) h(-1) H2O2、UV-A进行480分钟的光催化氧化，在颅内植入感染263K朊病毒株的不锈钢丝后，金黄叙利亚仓鼠的存活率为100%。有趣的是，在相同实验条件下，对感染263K的钛丝进行光催化处理显著延长了存活期，但未能消除传染性，我们将这一结果与相较于不锈钢，PrP(Sc)在钛上的吸附增加相关联。我们的研究结果有力地表明，我们这种对用户和环境友好的方案可安全应用于朊病毒感染的不锈钢表面的去污处理。
