Department of Biochemistry, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., United Kingdom.
Plant Cell Rep. 1986 Feb;5(1):72-6. doi: 10.1007/BF00269723.
The regeneration of protoplasts from potato (Solanum tuberosum L.) cvs. Desiree and King Edward has been significantly improved. Different shoot culture media were required for the release of viable protoplasts from cvs. Maris Piper and Desiree, and the response of protoplasts to different culture conditions depended upon the cultivar genotype of the protoplast source. Using protoplast isolation media containing 6mM CaCl2 improved protoplast viability and culture in enriched media lead to the reproducible and relatively efficient recovery of colonies from protoplasts of these cultivars. Over 70% of protoplast-derived calli from King Edward and Desiree regenerated shoots. Many shoots were grown to mature plants in soil. This is the first report of the regeneration of mature Desiree plants from protoplasts.
原生质体再生从马铃薯（Solanum tuberosum L.） cvs. Desiree 和 King Edward 已得到显著改善。从 Maris Piper 和 Desiree cvs 释放有活力的原生质体需要不同的芽培养培养基，而原生质体对不同培养条件的反应取决于原生质体来源的品种基因型。使用含有 6mM CaCl2 的原生质体分离培养基可提高原生质体的活力，在富含培养基中的培养可从这些品种的原生质体中重复且相对高效地回收菌落。超过 70%的 King Edward 和 Desiree 原生质体衍生的愈伤组织再生出芽。许多芽在土壤中生长为成熟植物。这是首次从原生质体再生出成熟的 Desiree 植株的报道。
