ENEA, Divisione Tecnologie Biologiche e Agrarie, C.R.E. Casaccia, I-00060, Rome, Italy.
Plant Cell Rep. 1986 Aug;5(4):243-6. doi: 10.1007/BF00269812.
In view of an evaluation of the relative efficiency of different in vitro systems for mutant induction and isolation in potato, a procedure of plant regeneration from protoplasts of some potato (Solanum tuberosum L.) cultivars was developed. Four cultivars (Primura, Kennebec, Spunta, Desirée) were used for isolation, culture and regeneration of leaf mesophyll protoplasts.These lines were chosen because of their economic importance in Italy and in the case of cv. Desirée for the presence of markers useful in the morphological characterization of regenerants.
鉴于对不同体外系统在马铃薯突变体诱导和分离方面的相对效率进行评估，开发了一种从一些马铃薯（Solanum tuberosum L.）品种的原生质体再生植株的方法。使用了四个品种（Primura、Kennebec、Spunta、Desirée）来分离、培养和再生叶片叶肉原生质体。选择这些系是因为它们在意大利具有经济重要性，而对于 cv. Desirée，则是因为存在有用的标记物，可用于再生体的形态特征描述。
