Alberta Research Council, 11315-87 Avenue, T6G 2C2, Edmonton, Alberta, Canada.
Plant Cell Rep. 1986 Feb;5(1):77-80. doi: 10.1007/BF00269724.
Ten cultivars and breeding lines from two species of alfalfa (Medicago media and M. sativa) were screened for their ability to produce embryos and plantlets from the root and hypocotyl under three different tissue culture protocols. The three protocols differed in basal salt composition, vitamins, hormones and cytokinin additions. That protocol having a high 2-4,D low cytokinin induction step gave the highest percentage of embryogenic calli in some cultivars and lines. M. media cultivars and breeding lines had a high percentage of embryoid formation. M. sativa cultivars gave no embryoid formation. Two M. media breeding lines (Br1 and Le1), which were intermediate in the percentage of embryogenic calli formed from explants, had the highest number of regenerated plants established in soil. The creeping rooted M. media cultivar Heinrichs produced the highest percentage of embryogenic calli from explants but most of these embryoids were abnormal and failed to grow in soil or vermiculite. Accordingly, successful regeneration is directly related to the quality and quantity of the embryoids produced.
对来自两种紫花苜蓿（Medicago media 和 M. sativa）的 10 个品种和品系进行了筛选，以研究它们在三种不同的组织培养方案下从根和下胚轴产生胚胎和小植株的能力。这三种方案在基本盐组成、维生素、激素和细胞分裂素添加物方面存在差异。在高 2-4，D 低细胞分裂素诱导步骤的方案中，一些品种和品系的胚性愈伤组织比例最高。M. media 品种和品系有很高的胚状体形成率。M. sativa 品种没有胚状体形成。两个 M. media 品系（Br1 和 Le1），从外植体形成的胚性愈伤组织的比例中等，在土壤中建立的再生植株数量最多。匍匐生根的 M. media 品种 Heinrichs 从外植体中产生了最高比例的胚性愈伤组织，但大多数胚状体异常，无法在土壤或珍珠岩中生长。因此，成功的再生直接与产生的胚状体的质量和数量有关。
