Department of Botany, The M.S. University, 390002, Baroda, India.
Plant Cell Rep. 1986 Jun;5(3):190-1. doi: 10.1007/BF00269115.
Somatic embryogenesis and subsequent formation of plantlets was obtained from callus cultures derived from leaves of mature (over 60years old) Soapnut (Sapindus trifoliatus L.) tree. Callus was induced from leaf explants and grown on Murashige and Skoog's medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) and kinetin. Reduction of 2,4-D concentration during subsequent subcultures resulted in formation of embryoids. These embryoids developed further when transferred to a medium containing benzylaminopurine and kinetin and then to a hormone-free medium. Unless 5-methyl tryptophan was added and the level of sucrose raised, the embryoids began to recallus and failed to form plantlets.
体细胞胚胎发生和随后的小植株形成是从成熟（超过 60 年）皂角（无患子）树叶片衍生的愈伤组织培养物中获得的。从叶片外植体诱导愈伤组织，并在补充有 2,4-二氯苯氧乙酸（2,4-D）和激动素的 Murashige 和 Skoog 培养基上生长。在随后的继代培养中降低 2,4-D 浓度会导致胚状体的形成。当将这些胚状体转移到含有苯丙氨酸和激动素的培养基上，然后转移到不含激素的培养基上时，胚状体会进一步发育。除非添加 5-甲基色氨酸并提高蔗糖水平，否则胚状体会开始再分化为愈伤组织，无法形成小植株。
