Faculty of Pharmaceutical Sciences, Kyoto University Yoshida, Sakyo-ku, 606, Kyoto, Japan.
Plant Cell Rep. 1986 Aug;5(4):310-3. doi: 10.1007/BF00269830.
Protoplasts prepared from an embryogenic cell suspension culture of fennel gave rise to somatic embryoids directly through unequal cell divisions of enlarged, ellipsoidal cells, when embedded in hormone-free LS agarose medium. On the other hand, protoplasts embedded in LS agarose medium containing 2,4-D and kinetin proliferated through unpolarized cell divisions to form calli, which gave somatic embryoids on the surface upon transfer onto the same medium. In either case, somatic embryoids germinated to develop into normal plantlets when cultured on hormone-free LS agar medium under illumination.
从茴香胚性细胞悬浮培养物中制备的原生质体，当嵌入无激素 LS 琼脂糖培养基中时，通过增大的椭圆形细胞的不均匀细胞分裂，直接产生体细胞胚状体。另一方面，嵌入含有 2,4-D 和激动素的 LS 琼脂糖培养基中的原生质体通过非极性细胞分裂增殖形成愈伤组织，当转移到相同的培养基上时，愈伤组织表面会产生体细胞胚状体。在这两种情况下，体细胞胚状体在光照下于无激素 LS 琼脂培养基上培养时，会发芽并发育成正常的小植株。
