Plant Research Laboratory, School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India.
Plant Cell Rep. 1986 Oct;5(5):322-4. doi: 10.1007/BF00268591.
Calli from young embryos of Cocos nucifera L. were induced on B5 medium supplemented with IAA-conjugates (IAA-asp or IAA-ala) at a concentration of 2.0 mg/1 and callusing was increased by about 10% if both IAA-conjugates, IAA-asp and IAA-ala were added together. Differentiation of shoots and roots was achieved by transferring calli to B5 medium supplemented with either IAA-asp (2.0 mg/1)+Kn(2.0 mg/1) or NAA (2.0 mg/1). Complete plantlets were obtained on B5 medium supplemented with NAA (0.5 mg/1)+BAP (2.0 mg/1)+PVP (1.0 g/1).
在 B5 培养基中添加 2.0mg/L 的 IAA 缀合物（IAA-asp 或 IAA-ala），可诱导椰子 Calli 形成愈伤组织，添加两种 IAA 缀合物（IAA-asp 和 IAA-ala）可使愈伤组织的形成增加约 10%。通过将愈伤组织转移到添加 IAA-asp（2.0mg/L）+Kn（2.0mg/L）或 NAA（2.0mg/L）的 B5 培养基中，实现了芽和根的分化。在添加 NAA（0.5mg/L）+BAP（2.0mg/L）+PVP（1.0g/L）的 B5 培养基上获得完整的植株。
