School of Pharmaceutical Engineering, Jiangsu Food and Pharmaceutical Science College, Huai'an, 223003, Jiangsu, China.
Jiangsu Engineering Laboratory of Protein Drugs, Huai'an, 223003, Jiangsu, China.
Curr Microbiol. 2019 Jun;76(6):706-712. doi: 10.1007/s00284-019-01681-0. Epub 2019 Apr 8.
It is well known that cofactors play a key role in the production of different compounds in bioconversion processes, while the high cost of cofactors limits their usage in industrial applications. In the present study, a NADH regeneration system was successfully developed in Lactobacillus plantarum by expressing the fdh gene coding for formate dehydrogenase (FDH) from Candida boidinii. Results indicated that the FDH was expressed with the highest activity of 0.82 U/mg of protein when cells entered early stationary phase. In addition, the expression of FDH increased the intracellular level of NADH and NADH/NAD ratio in L. plantarum, and therefore, enhanced the NADH-dependent production of 3-phenyllactic acid (PLA) in repeated and fed-batch bioconversions. In brief, the results demonstrate that the NADH regeneration by expressing FDH is a promising strategy for producing NADH-dependent microbial metabolites in L. plantarum.
众所周知，辅因子在生物转化过程中不同化合物的生产中起着关键作用，而辅因子的高成本限制了它们在工业应用中的使用。在本研究中，通过表达来自 Candida boidinii 的甲酸脱氢酶（FDH）基因，成功在植物乳杆菌中开发了 NADH 再生系统。结果表明，当细胞进入早期静止期时，FDH 的表达活性最高，为 0.82 U/mg 蛋白。此外，FDH 的表达增加了植物乳杆菌细胞内 NADH 的水平和 NADH/NAD 比，从而增强了 NADH 依赖性 3-苯乳酸（PLA）在重复和分批补料生物转化中的生产。总之，这些结果表明，通过表达 FDH 进行 NADH 再生是在植物乳杆菌中生产 NADH 依赖性微生物代谢物的一种很有前途的策略。
