Ralfkiaer E, Pulford K A, Gatter K C, Wantzin G L, Mason D Y
J Clin Pathol. 1986 May;39(5):547-52. doi: 10.1136/jcp.39.5.547.
The reactivity of a monoclonal antibody against human T lymphotropic retrovirus (antibody 12/1-2, recognising the HTLV-1 p19 internal core viral protein) with benign and malignant cutaneous biopsy specimens was examined and compared with results obtained on normal skin, on various other human cells and tissues, and on immunoblotted extracts of tonsil squamous epithelium. In keeping with previous studies, 12/1-2 labelled a proportion of the thymic epithelial stroma and the entire layer of basal cells in stratified non-keratinized and keratinized epithelium. Furthermore, antibody 12/1-2 reacted with basal cell carcinomas and showed an essentially identical staining pattern in normal skin, cutaneous T cell lymphomas, and a range of benign dermatoses. The dot blot preparations showed that 12/1-2 recognised an antigen associated with keratin intermediate filaments. These data indicate that antibody 12/1-2 forms a useful marker for subsets of epithelial cells, which presumably participate in T cell education, and that a range of cutaneous disorders of widely different aetiology show no abnormalities in epithelial expression of this antigen.
检测了一种抗人嗜T淋巴细胞逆转录病毒的单克隆抗体（抗体12/1 - 2，识别HTLV - 1 p19内部核心病毒蛋白）与良性和恶性皮肤活检标本的反应性，并将结果与在正常皮肤、各种其他人类细胞和组织以及扁桃体鳞状上皮免疫印迹提取物上获得的结果进行比较。与先前的研究一致，12/1 - 2标记了一部分胸腺上皮基质以及分层的非角化和角化上皮中的整个基底细胞层。此外，抗体12/1 - 2与基底细胞癌反应，并在正常皮肤、皮肤T细胞淋巴瘤和一系列良性皮肤病中显示出基本相同的染色模式。斑点印迹制剂表明，12/1 - 2识别一种与角蛋白中间丝相关的抗原。这些数据表明，抗体12/1 - 2是上皮细胞亚群的有用标志物，推测这些上皮细胞参与T细胞的发育，并且一系列病因广泛不同的皮肤疾病在该抗原的上皮表达上没有异常。
