Ponder B A, Wilkinson M M
J Histochem Cytochem. 1981 Aug;29(8):981-4. doi: 10.1177/29.8.7024402.
In mammals there are two forms of alkaline phosphatase, one of which is widely distributed in a variety of tissues, and one of which is confined to intestine. Levamisole (1-tetramisole) inhibits the nonintestinal form of the enzyme, but is without effect on the intestinal form. We have exploited this difference by using conjugates made with calf intestinal alkaline phosphatase for immunohistochemical demonstration of H2 antigens in frozen section of mouse tissues. The alkaline phosphatase staining is performed in the presence of 1 mm levamisole, which inhibits the endogenous tissue enzyme without loss of staining by the conjugate. Endogenous enzyme can be inhibited by other means, such as exposure to 20% acetic acid, but labile antigens may be destroyed.
在哺乳动物中，碱性磷酸酶有两种形式，一种广泛分布于多种组织中，另一种则局限于肠道。左旋咪唑（1-四咪唑）可抑制该酶的非肠道形式，但对肠道形式无作用。我们利用了这种差异，通过使用与小牛肠道碱性磷酸酶制成的结合物，对小鼠组织冰冻切片中的H2抗原进行免疫组织化学显示。碱性磷酸酶染色在1 mM左旋咪唑存在的情况下进行，它可抑制内源性组织酶，而不影响结合物的染色。内源性酶可用其他方法抑制，如暴露于20%的乙酸中，但可能会破坏不稳定的抗原。
