Chu A C, Patterson J A, Goldstein G, Berger C L, Takezaki S, Edelson R L
J Invest Dermatol. 1983 Sep;81(3):194-7. doi: 10.1111/1523-1747.ep12517686.
A heterologous antithymopoietin (anti-TP) antibody was used to determine whether a TP-like molecule is present in the epidermis, since such factors have been postulated to play a part in known T cell-epidermal cell interaction. Examination of cytocentrifuge smears of freshly separated human epidermal cells stained by indirect immunofluorescence revealed that 8-14% of these cells possessed cytoplasmic reactivity with the anti-TP antibody. Similarly, 2-5% of human epidermal cells, maintained in tissue culture for 2-8 weeks, showed cytoplasmic staining with the anti-TP antibody. Double-labeling immunofluorescence studies, with the anti-TP antibody and a monoclonal antibody specifically reactive with Langerhans cells (OKT6), demonstrated that cells possessing this TP-like substance were not Langerhans cells. In situ studies of 4-microns frozen sections of normal human skin indicated that the cell population which possesses the TP-like substance is the basal layer of keratincoytes in the epidermis.
由于推测这类因子在已知的T细胞与表皮细胞相互作用中发挥作用，因此使用了一种异源抗胸腺生成素（anti-TP）抗体来确定表皮中是否存在类似TP的分子。通过间接免疫荧光对新鲜分离的人表皮细胞的细胞离心涂片进行检测，结果显示，这些细胞中有8%-14%的细胞与抗TP抗体发生细胞质反应。同样，在组织培养中维持2-8周的人表皮细胞中有2%-5%的细胞显示出与抗TP抗体的细胞质染色。使用抗TP抗体和与朗格汉斯细胞特异性反应的单克隆抗体（OKT6）进行双标记免疫荧光研究表明，拥有这种类似TP物质的细胞不是朗格汉斯细胞。对正常人皮肤4微米冰冻切片的原位研究表明，拥有类似TP物质的细胞群体是表皮中角质形成细胞的基底层。
