Boylston A W, Borst J, Yssel H, Blanchard D, Spits H, de Vries J E
J Immunol. 1986 Jul 15;137(2):741-4.
Five Mab raised against the T cell antigen receptor of the human T cell line HPB-ALL which react with a subpopulation of normal peripheral blood T cells are described. Three Mab, 3D6, 1C1, and 1C2, react with 3 to 5% of normal PBL and stimulate proliferation of the cells with which they react. An increase in the number of cells which react with all five Mab occurs. Two Mab, 2D4 and 65, react with subsets of the cells which bind 1C1, 1C2, and 3D6 and divide the family into four subgroups, 2D4+ 65+, 2D4+ 65-, 2D4- 65+, and 2D4- 65-. Functional T cell clones in all four subfamilies have been observed. Cytolytic function can be correlated with the TcR phenotype expressed because all of the Mab which react with a particular clone inhibit its ability to lyse a specific target. The epitopes recognized by the panel are closely related because all five block each other's binding to HPB-ALL. In addition, the determinants recognized by 3D6, 1C1, and 1C2 on normal lymphocytes are probably very closely related because all clones examined react with all three Mab.
本文描述了五种针对人T细胞系HPB - ALL的T细胞抗原受体产生的单克隆抗体（Mab），它们可与正常外周血T细胞的一个亚群发生反应。三种单克隆抗体，即3D6、1C1和1C2，可与3%至5%的正常外周血淋巴细胞（PBL）发生反应，并刺激与之反应的细胞增殖。与所有五种单克隆抗体发生反应的细胞数量增加。另外两种单克隆抗体，2D4和65，可与结合1C1、1C2和3D6的细胞亚群发生反应，并将该细胞群分为四个亚组，即2D4 + 65 +、2D4 + 65 -、2D4 - 65 +和2D4 - 65 -。在所有四个亚家族中均观察到了功能性T细胞克隆。细胞溶解功能可与所表达的T细胞受体（TcR）表型相关，因为与特定克隆发生反应的所有单克隆抗体均会抑制其裂解特定靶标的能力。该组单克隆抗体所识别的表位密切相关，因为所有五种单克隆抗体均可阻断彼此与HPB - ALL的结合。此外，3D6、1C1和1C2在正常淋巴细胞上所识别的决定簇可能非常密切相关，因为所有检测的克隆均与所有这三种单克隆抗体发生反应。
