Liu Zhen, Gao Wenyuan, Zhang Jingze, Hu Jing
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Iran J Pharm Res. 2012 Spring;11(2):611-20.
The seed of Croton tiglium L. (SCT) is a well known folk medicine. In China, it has used to treat gastrointestinal disorders, intestinal inflammation, rheumatism, and so on. Previous studies established its purgative and inflammation properties. In addition, the effects of essential oil of SCT on intestinal transit and gastrointestinal tract has been studied. In the present study, we evaluated the antinociceptive effect of SCT through the writhing test in mice, investigated the effects of it on spontaneous smooth muscle contractions of isolated rabbit jejunum and examined the in-vitro results through the in-vivo small intestine propulsion. We further investigated the possible compounds using HPLC-MS, and six compounds were tentatively identified as phorbol esters. Furthermore, the possible fragmentation pathways of phorbol esters were proposed, and we also detected the possible compounds in the active parts.
巴豆（Croton tiglium L.）的种子是一种著名的民间药物。在中国，它被用于治疗胃肠道疾病、肠道炎症、风湿病等。先前的研究证实了其泻下和抗炎特性。此外，还研究了巴豆种子精油对肠道转运和胃肠道的影响。在本研究中，我们通过小鼠扭体试验评估了巴豆种子的镇痛作用，研究了其对离体兔空肠自发平滑肌收缩的影响，并通过体内小肠推进试验检验了体外实验结果。我们进一步使用高效液相色谱 - 质谱联用仪（HPLC-MS）研究了可能的化合物，初步鉴定出六种化合物为佛波酯。此外，还提出了佛波酯可能的裂解途径，并且我们还检测了活性部位中的可能化合物。
