Department of Crop Science, Faculty of Agriculture, 43400 UPM Serdang, Selangor, Pakistan.
BMC Complement Altern Med. 2014 Feb 22;14:71. doi: 10.1186/1472-6882-14-71.
Isodon rugosus is used in folk Pakistan traditional practices to cure ailments related to gastrointestinal, respiratory and cardiovascular problems. Present study was undertaken to validate these folkloric uses.
A crude methanol extract of the aerial parts of Isodon rugosus (Ir.Cr.) was used for both in vitro and in vivo experiments. The plant extract was tested on isolated rabbit jejunum preparations for possible presence of spasmolytic activity. Moreover, isolated rabbit tracheal and aorta preparations were used to ascertain the relaxant effects of the extract. Acetylcholinesterase and butyrylcholinesterase inhibitory activities of Ir.Cr were also determined as well as its antioxidant activity. The in vivo antiemetic activity of the extract was evaluated by using the chick emesis model, while the analgesic and antipyretic activities were conducted on albino mice.
The application of the crude extract of I. rugosus to isolated rabbit jejunum preparations exhibited relaxant effect (0.01-0.3 mg/ml). The Ir.Cr also relaxed K+(80 m M)-induced spastic contractions in isolated rabbit jejunum preparations and shifted the Ca+2 concentration response curves towards right (0.01-0.3 mg/ml). Similarly, the extract, when applied to the isolated rabbit tracheal preparations relaxed the carbachol (1 μM)--as well as K+ (80 mM)-induced contractions in a concentration range of 0.01-1.0 mg/ml. Moreover, it also relaxed (0.01-3.0 mg/ml) the phenylephrine (1 μM)- and K+ (80 mM)-induced contractions in isolated rabbit aorta preparations. The Ir.Cr (80 mg/kg) demonstrated antipyretic activity on pyrogen-induced pyrexia in rabbits as compared to aspirin as standard drug. The Ir.Cr also exhibited anti-oxidant as well as inhibitory effect on acetyl- and butyryl-cholinesterase and lipoxygenase (0.5 mg/ml).
The observed relaxant effect on isolated rabbit jejunum, trachea and aorta preparations caused by Ir.Cr is possibly to be mediated through Ca+2 channel blockade and therefore may provided scientific basis to validate the folkloric uses of the plant in the management of gastrointestinal, respiratory and cardiovascular ailments. The observed antioxidant activity as well as the lipoxygenase inhibitory activity may validate its traditional use in pain and inflammations.
糙苏在巴基斯坦民间传统医学中被用于治疗与胃肠道、呼吸道和心血管问题相关的疾病。本研究旨在验证这些民间用途。
采用糙苏（Ir.Cr.）地上部分的甲醇粗提物进行体内外实验。将植物提取物应用于离体兔空肠制备物，以检测可能存在的平滑肌松弛活性。此外，还使用离体兔气管和主动脉制备物来确定提取物的舒张作用。还测定了 Ir.Cr 的乙酰胆碱酯酶和丁酰胆碱酯酶抑制活性以及抗氧化活性。通过使用雏鸡呕吐模型评估提取物的体内止吐活性，同时在白化小鼠上进行镇痛和退热活性研究。
糙苏粗提取物应用于离体兔空肠制备物显示出松弛作用（0.01-0.3mg/ml）。Ir.Cr 还可松弛 K+（80mM）诱导的离体兔空肠制备物痉挛收缩，并使 Ca+2浓度反应曲线向右移位（0.01-0.3mg/ml）。同样，当应用于离体兔气管制备物时，该提取物可松弛 carbachol（1μM）-以及 K+（80mM）诱导的收缩，浓度范围为 0.01-1.0mg/ml。此外，它还可松弛（0.01-3.0mg/ml）苯肾上腺素（1μM）-和 K+（80mM）诱导的离体兔主动脉制备物收缩。与标准药物阿司匹林相比，Ir.Cr（80mg/kg）在发热兔中表现出退热活性。Ir.Cr 还表现出抗氧化以及对乙酰胆碱酯酶和丁酰胆碱酯酶和脂氧合酶的抑制作用（0.5mg/ml）。
Ir.Cr 对离体兔空肠、气管和主动脉制备物的松弛作用可能是通过 Ca+2通道阻断介导的，因此可能为验证该植物在胃肠道、呼吸道和心血管疾病治疗中的民间用途提供科学依据。观察到的抗氧化活性以及脂氧合酶抑制活性可能验证其在疼痛和炎症中的传统用途。
