Salavati Parvin, Ramezani Mina, Monsef-Esfahani Hamid R, Hajiagha Reza, Parsa Maliheh, Tavajohi Shoreh, Ostad Seyed Nasser
Department of Animal Science, Faculty of Biology, University of Payam Noor, Tehran, Iran.
Iran J Pharm Res. 2013 Spring;12(2):389-94.
Neuroprotective effect of the extract from aerial parts of Scrophularia striata Boiss (Scrophulariaceae) was investigated against glutamate-induced neurotoxicity on cultured rat pups Cerebellar Granule Neurons (CGNs). CGNs from 8 days old Sprague-Dawley rat were prepared and cultured. The experiments were performed after 8 days in culture. The plant was collected from the northeastern part (Ruin region) of Iran and air-dried at room temperature. The total extract was prepared with maceration of prepared powder in ethanol 80% for three times. SEQUENTIAL EXTRACTS WERE OBTAINED USING DRIED AND POWDERED AERIAL PARTS WITH INCREASINGLY POLAR SOLVENTS: petroleum ether, chloroform, ethyl acetate and methanol 80% solution. Cultured cells were exposed to 125 μM of glutamate for 12 h following a 24 h of incubation with test fractions at concentration of 10 mcg/mL. Morphological assay was performed using invert light microscope after fixation and staining with haematoxylin. Neuronal viability was measured using MTT assay. Statistical analysis was done using SPSS software. One way analysis of variance (ANOVA) was performed by Tukey post-hoc test. Values were considered statistically significant when p-value ≤ 0.05. Results of this study showed a significant neuroprotective activity of high polarity methanolic fraction of aerial parts of Scrophularia striata against glutamate-induced neurotoxicity in a dosedependent manner. Treatment with 10 mcg/mL of the fractions showed the best result.
研究了玄参科条纹玄参地上部分提取物对谷氨酸诱导的原代培养大鼠小脑颗粒神经元（CGNs）神经毒性的神经保护作用。制备并培养了8日龄Sprague-Dawley大鼠的CGNs。培养8天后进行实验。该植物采自伊朗东北部（鲁因地区），室温下风干。将制备好的粉末用80%乙醇浸渍三次制备总提取物。使用干燥并粉碎的地上部分，依次用极性递增的溶剂：石油醚、氯仿、乙酸乙酯和80%甲醇溶液获得连续提取物。在浓度为10 mcg/mL的测试组分孵育24小时后，将培养的细胞暴露于125 μM谷氨酸中12小时。固定并用苏木精染色后，使用倒置光学显微镜进行形态学分析。使用MTT法测量神经元活力。使用SPSS软件进行统计分析。采用Tukey事后检验进行单因素方差分析（ANOVA）。当p值≤0.05时，认为差异具有统计学意义。本研究结果表明，条纹玄参地上部分的高极性甲醇组分对谷氨酸诱导的神经毒性具有显著的剂量依赖性神经保护活性。用10 mcg/mL的该组分处理显示出最佳效果。
