Hajiaghaee Reza, Monsef-Esfahani Hamid R, Khorramizadeh Mohammad R, Saadat Farshid, Shahverdi Ahmad R, Attar Farideh
Department of Pharmacognosy, Faculty of Pharmacy, Medical Sciences, University of Tehran, Tehran, Iran.
Phytother Res. 2007 Dec;21(12):1127-9. doi: 10.1002/ptr.2221.
It has been shown that destruction of the extracellular matrix (ECM) by matrix metalloproteinases (MMPs) leads to tumor invasion, metastasis and angiogenesis. The aim of the present research was to verify the effects of a total extract and sequential extracts of Scrophularia striata Boiss (Scrophulariaceae) on MMPs activity. The fibro sarcoma cell line (wehi-164) was used to assess MMPs inhibitory effect. The MMPs activity was evaluated using a zymoanalysis method. The results of this study showed that the highest MMPs inhibitory effect (55.6%) was observed after treatment with 10 microg/mL of the high polarity methanol solution extract corresponding to its lowest cytotoxicity effect (4%). It is concluded that the aerial parts of S. striata might contain various polar compounds that inhibit MMPs.
研究表明，基质金属蛋白酶（MMPs）对细胞外基质（ECM）的破坏会导致肿瘤侵袭、转移和血管生成。本研究的目的是验证玄参（玄参科）总提取物和序贯提取物对MMPs活性的影响。使用纤维肉瘤细胞系（wehi - 164）评估MMPs抑制作用。采用酶谱分析方法评估MMPs活性。本研究结果表明，用10微克/毫升高极性甲醇溶液提取物处理后，观察到最高的MMPs抑制作用（55.6%），同时其细胞毒性作用最低（4%）。得出的结论是，玄参地上部分可能含有多种抑制MMPs的极性化合物。
